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April 2015

Volume 2  Issue 1
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Research Article
Analysis of the Correlation between Oxacillin/Cefoxitin Susceptibility/Resistance, the Presence of the Gene MecA and PBP2a Production in Hospital Infection-Associated Staphylococcal Isolates

 Eduarda Vanessa Cavalcante Mangueira, Wagner Luis Mendes de Oliveira, Alzira Maria Paiva de Almeida, and Nilma Cintra Leal*

Oxacillin/cefoxitin-susceptible mecA-positive staphylococcal strains present a challenge for clinical and therapeutic management and require great attention from clinical microbiology laboratories, as phenotypical tests for oxacillin/cefoxitin and PBP2a detection might be misleading, and the incorrect identification could lead to treatment with inappropriate antimicrobial therapy. PBP2a/PBP2´ is an alternative, low-affinity penicillin-binding protein (PBP) encoded by the gene mecA.

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Research Article
Canine Distemper Virus with Concomitant Infections Due to Canine Herpesvirus-1, Canine Parvovirus, and Canine Adenovirus in Puppies from Southern Brazil

Selwyn A. Headley*, Livia Bodnar, Ana P. Silva, Alice F. Alfieri, Lucas A. Gomes, Werner Okano, Amauri A. Alfieri

 The clinical, pathological, and molecular findings associated with mortalities due canine distemper virus (CDV) with concomitant infections of Canid herpesvirus type 1 (CaHV-1), Canid adenovirus type 2 (CAdV-2), and canine parvovirus type 2 (CPV-2) are described in puppies from southern Brazil. Four 3-8 months-old, German Spitz, puppies (1, female; 3, males) with clinical manifestations of abdominal pain, extreme vocalization, convulsions, and icterus died suddenly and were necropsied. Significant pathological alterations included necrohemorrhagic hepatitis, necrohemorrhagic nephritis, disseminated vasculitis, pulmonary hemorrhage with necrotizing bronchitis and/or bronchiolitis, non-suppurative myocarditis, and hemorrhagic enteritis.  

 

                                                                                                                                                                                                                           Full text   Download PDF

 

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 Research Article
Molecular Characterization of Escherichia coli and Klebsiella pneumoniae Producing Extended-Spectrum Beta-Lactamases (ESBL’s) Isolated in Two Hospitals in Murcia (Spain)

 Cristina Casañ*, Carme Salvador, Nuria Tormo, Carmen Guerrero, Concepción Gimeno, Manuel Segovia and Genoveva Yagüe

The increase in clinical isolation of extended-spectrum β-lactamases (ESBLs) in Enterobacteriaceae entails an important worldwide problem because they are resistant to a large number of beta-lactams, but also because they are frequently found in genetic mobile elements in coexistence with genes that confer resistance to other important groups of antimicrobial drugs, thus leading to the restriction in therapeutic options.

 

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